Digital transcriptomics: A flavour of SAGE | Zendy

Kåre Lehmann Nielsen | Zendy; Jeppe Emmersen | Zendy; Karen G. Welinder | Zendy

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Digital transcriptomics: A flavour of SAGE

Author(s) -

Kåre Lehmann Nielsen,

Jeppe Emmersen,

Karen G. Welinder

Publication year - 2004

Publication title -

the biochemist

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.126

H-Index - 7

eISSN - 1740-1194

pISSN - 0954-982X

DOI - 10.1042/bio02604021

Subject(s) - serial analysis of gene expression , transcriptome , dna microarray , complementary dna , sage , biology , gene , computational biology , genetics , gene expression , microarray , physics , nuclear physics

Transcriptome analyses compare the expression level of all or a selected set of genes in an organism in response to various environmental conditions in a broad sense. SAGE (serial analysis of gene expression) is a digital transcriptomics method complementary to hybridizationbased methods such as DNA microarrays. SAGE counts the number of 21 nt tags cut out from each cDNA as a measure of expression of the corresponding mRNA, known as well as unknown. Furthermore, the tags can be used to RACE (rapid amplification of cDNA ends)-clone the unknown genes and to verify predicted exons.

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