DNAzymes for amine and peptide lysine acylation | Zendy

Tianjiong Yao | Zendy; Jack J. Przybyla | Zendy; Peter Z. Yeh | Zendy; Austin M. Woodard | Zendy; Hannah J. Nilsson | Zendy; Benjamin M. Brandsen | Zendy; Scott Silverman | Zendy

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DNAzymes for amine and peptide lysine acylation

Author(s) -

Tianjiong Yao,

Jack J. Przybyla,

Peter Z. Yeh,

Austin M. Woodard,

Hannah J. Nilsson,

Benjamin M. Brandsen,

Scott Silverman

Publication year - 2020

Publication title -

organic and biomolecular chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 146

eISSN - 1477-0539

pISSN - 1477-0520

DOI - 10.1039/d0ob02015j

Subject(s) - acylation , lysine , deoxyribozyme , chemistry , peptide , amino acid , amine gas treating , biochemistry , combinatorial chemistry , stereochemistry , biology , dna , organic chemistry , catalysis

DNAzymes were previously identified by in vitro selection for a variety of chemical reactions, including several biologically relevant peptide modifications. However, finding DNAzymes for peptide lysine acylation is a substantial challenge. By using suitably reactive aryl ester acyl donors as the electrophiles, here we used in vitro selection to identify DNAzymes that acylate amines, including lysine side chains of DNA-anchored peptides. Some of the DNAzymes can transfer a small glutaryl group to an amino group. These results expand the scope of DNAzyme catalysis and suggest the future broader applicability of DNAzymes for sequence-selective lysine acylation of peptide and protein substrates.

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