Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid–liquid extraction and surface enhanced Raman scattering | Zendy

Lidia Morelli | Zendy; Laura Serioli | Zendy; Francesca Alessandra Centorbi | Zendy; Christian Bille Jendresen | Zendy; M. Matteucci | Zendy; Oleksii Ilchenko | Zendy; Danilo Demarchi | Zendy; Alex Toftgaard Nielsen | Zendy; Kinga Zór | Zendy; Anja Boisen | Zendy

Open Access

Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid–liquid extraction and surface enhanced Raman scattering

Author(s) -

Lidia Morelli,

Laura Serioli,

Francesca Alessandra Centorbi,

Christian Bille Jendresen,

M. Matteucci,

Oleksii Ilchenko,

Danilo Demarchi,

Alex Toftgaard Nielsen,

Kinga Zór,

Anja Boisen

Publication year - 2018

Publication title -

lab on a chip

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.064

H-Index - 210

eISSN - 1473-0197

pISSN - 1473-0189

DOI - 10.1039/c7lc01217a

Subject(s) - extraction (chemistry) , materials science , raman scattering , liquid liquid , chromatography , liquid–liquid extraction , analytical chemistry (journal) , raman spectroscopy , chemistry , optics , physics

We present the development of an automated centrifugal microfluidic platform with integrated sample pre-treatment (filtration and liquid-liquid extraction) and detection (SERS-based sensing). The platform consists of eight calibration and four assay modules, fabricated with polypropylene using injection molding and bonded with ultrasonic welding. The platform was used for detection of a secondary bacterial metabolite (p-coumaric acid) from bacterial supernatant. The obtained extraction efficiency was comparable to values obtained in batch experiments and the SERS-based sensing showed a good correlation with HPLC analysis.

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