The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair | Zendy

Elena Eremeeva | Zendy; Mikhail Abramov | Zendy; Philippe Marlière | Zendy; Piet Herdewijn | Zendy

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The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair

Author(s) -

Elena Eremeeva,

Mikhail Abramov,

Philippe Marlière,

Piet Herdewijn

Publication year - 2016

Publication title -

organic and biomolecular chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 146

eISSN - 1477-0539

pISSN - 1477-0520

DOI - 10.1039/c6ob02274j

Subject(s) - chemistry , duplex (building) , deoxyuridine , deoxyadenosine , polymerase , base pair , dna , taq polymerase , stereochemistry , dna polymerase , microbiology and biotechnology , biochemistry , biology , thermus aquaticus

5-Chloro-2'-deoxyuridine as a possible component of a chemically modified genome has been discussed in terms of its influence on duplex stability and DNA polymerase incorporation properties. The search for its counterpart among different deoxyadenosine analogs (7-deaza-, 8-aza- and 8-aza-7-deaza-2'-deoxyadenosines) showed that the stable duplex formation as well as the synthesis of long constructs, more than 2 kb, were successful with the 5-chloro-2'-deoxyuridine and 7-deaza-2'-deoxyadenosine combination and with Taq DNA polymerase.

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