The dual aptamer approach: rational design of a high-affinity FAD aptamer | Zendy

Thomas Merkle | Zendy; Isabelle T. Holder | Zendy; Jörg S. Hartig | Zendy

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The dual aptamer approach: rational design of a high-affinity FAD aptamer

Author(s) -

Thomas Merkle,

Isabelle T. Holder,

Jörg S. Hartig

Publication year - 2015

Publication title -

organic and biomolecular chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 146

eISSN - 1477-0539

pISSN - 1477-0520

DOI - 10.1039/c5ob02026c

Subject(s) - aptamer , chemistry , combinatorial chemistry , dual (grammatical number) , rational design , computational biology , biochemistry , nanotechnology , microbiology and biotechnology , materials science , biology , art , literature

A design strategy for high-affinity aptamers of complex biomolecules is presented. We developed an RNA with FAD-binding properties by combining known ATP- and FMN-aptamers. Cooperative binding of FAD was shown by SPR spectroscopy and fluorescence assays. The strategy should be transferable to several other biomolecules.

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