Open AccessDNA polymerase-catalyzed incorporation of nucleotides modified with a G-quadruplex-derived DNAzyme
Author(s) -
Daniela Verga,
Mark W. Welter,
AnnaLena Steck,
Andreas Marx
Publication year - 2015
Publication title -
chemical communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.837
H-Index - 333
eISSN - 1364-548X
pISSN - 1359-7345
DOI - 10.1039/c5cc01387a
Subject(s) - deoxyribozyme , g quadruplex , nucleotide , polymerase , chemistry , catalysis , dna polymerase , dna , microbiology and biotechnology , combinatorial chemistry , biochemistry , biology , gene
We show that nucleotides which are modified with a G-quadruplex-derived DNAzyme are substrates for DNA polymerases. Based on this finding we developed a naked-eye detection system that allows the detection of single nucleotide variations in DNA.
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