Open AccessHigh-resolution NMR-based metabolic detection of microgram biopsies using a 1 mm HRμMAS probe
Author(s) -
Yusuke Nishiyama,
Yuki Endo,
Takahiro Nemoto,
AnneKarine BouzierSore,
Alan Wong
Publication year - 2015
Publication title -
the analyst
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 153
eISSN - 1364-5528
pISSN - 0003-2654
DOI - 10.1039/c5an01810b
Subject(s) - microgram , chemistry , resolution (logic) , chromatography , computer science , biochemistry , in vitro , artificial intelligence
A prototype 1 mm High-Resolution micro-Magic Angle Spinning (HRμMAS) probe is described. High quality (1)H NMR spectra were obtained from 490 μg of heterogeneous biospecimens, offering a rich-metabolite profiling. The results demonstrate the potential of HRμMAS as a new NMR analytical tool in metabolomics.
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