Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases | Zendy

Sevnur Serim | Zendy; Philipp Baer | Zendy; Steven H. L. Verhelst | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases

Author(s) -

Sevnur Serim,

Philipp Baer,

Steven H. L. Verhelst

Publication year - 2014

Publication title -

organic and biomolecular chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 146

eISSN - 1477-0539

pISSN - 1477-0520

DOI - 10.1039/c4ob02444c

Subject(s) - chemistry , phosphonate , aryl , alkyl , proteases , serine , fluorescence , organic chemistry , stereochemistry , enzyme , physics , quantum mechanics

Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research