eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA–gold affinity interactions | Zendy

Abu Ali Ibn Sina | Zendy; Sidney Howell | Zendy; Laura G. Carrascosa | Zendy; Sakandar Rauf | Zendy; Muhammad J. A. Shiddiky | Zendy; Matt Trau | Zendy

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eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA–gold affinity interactions

Author(s) -

Abu Ali Ibn Sina,

Sidney Howell,

Laura G. Carrascosa,

Sakandar Rauf,

Muhammad J. A. Shiddiky,

Matt Trau

Publication year - 2014

Publication title -

chemical communications

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.837

H-Index - 333

eISSN - 1364-548X

pISSN - 1359-7345

DOI - 10.1039/c4cc06732k

Subject(s) - dna , dna methylation , cpg site , electrochemistry , chemistry , methylation , electrode , resolution (logic) , microbiology and biotechnology , analytical chemistry (journal) , biophysics , chromatography , biochemistry , biology , gene , gene expression , artificial intelligence , computer science

We report a simple electrochemical method referred to as "eMethylsorb" for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.

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