A novel technique for the formation of embryoid bodies inside liquid marbles | Zendy

Fatemeh Sarvi | Zendy; Tina Arbatan | Zendy; Peggy Chan | Zendy; Wei Shen | Zendy

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A novel technique for the formation of embryoid bodies inside liquid marbles

Author(s) -

Fatemeh Sarvi,

Tina Arbatan,

Peggy Chan,

Wei Shen

Publication year - 2013

Publication title -

rsc advances

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.746

H-Index - 148

ISSN - 2046-2069

DOI - 10.1039/c3ra40364e

Subject(s) - embryoid body , particle (ecology) , chemistry , seeding , chemical engineering , materials science , nanotechnology , embryonic stem cell , biochemistry , oceanography , adult stem cell , aerospace engineering , engineering , gene , geology

The significant and inherent limitations associated with commonly used methods of in vitro embryoid body (EB) formation motivate the development of novel, facile, efficient and reproducible techniques. In this study we report the possibility of using "liquid marbles" as facile and efficient micro bioreactors for in vitro EB formation. To exploit liquid marbles as micro biological reactors, embryonic stem cells (ES cells) were inoculated into liquid marbles containing embryonic cell growth medium. Herein we show how the confined internal space of a liquid marble, along with the porous and non-adhesive properties of the highly hydrophobic liquid marble shell, can provide the necessary conditions for the formation of uniform EBs inside liquid marbles. Factors such as the powder particle size, the liquid marble volume and the cell seeding density inside each liquid marble were also investigated to evaluate the effects of varying experimental conditions on the efficiency of EB formation within a liquid marble

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