Site-selected incorporation of 5-carboxymethylaminomethyl(-2-thio)uridine into RNA sequences by phosphoramidite chemistry | Zendy

Grażyna Leszczyńska | Zendy; Jakub Pięta | Zendy; K. Woźniak | Zendy; Andrzej Małkiewicz | Zendy

Open Access

Site-selected incorporation of 5-carboxymethylaminomethyl(-2-thio)uridine into RNA sequences by phosphoramidite chemistry

Author(s) -

Grażyna Leszczyńska,

Jakub Pięta,

K. Woźniak,

Andrzej Małkiewicz

Publication year - 2013

Publication title -

organic and biomolecular chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 146

eISSN - 1477-0539

pISSN - 1477-0520

DOI - 10.1039/c3ob42302f

Subject(s) - phosphoramidite , chemistry , uridine , transfer rna , rna , thio , nucleic acid , stereochemistry , combinatorial chemistry , biochemistry , oligonucleotide , dna , gene

5-Carboxymethylaminomethyluridine (cmnm(5)U) and 5-carboxymethylaminomethyl-2-thiouridine (cmnm(5)s(2)U) are located at the wobble position in several cytosolic and mitochondrial tRNA sequences. In this paper, we report the first site-selected incorporation of cmnm(5)U and cmnm(5)s(2)U into RNA sequences by phosphoramidite chemistry on a CPG solid support. Trifluoroacetyl and 2-(trimethylsilyl)ethyl were selected for the protection of the amine and carboxyl functions, respectively.

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