Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation | Zendy

Laura G. Carrascosa | Zendy; Abu Ali Ibn Sina | Zendy; Ramkumar Palanisamy | Zendy; Borja Sepúlveda | Zendy; Marinus A. Otte | Zendy; Sakandar Rauf | Zendy; Muhammad J. A. Shiddiky | Zendy; Matt Trau | Zendy

Open Access

Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation

Author(s) -

Laura G. Carrascosa,

Abu Ali Ibn Sina,

Ramkumar Palanisamy,

Borja Sepúlveda,

Marinus A. Otte,

Sakandar Rauf,

Muhammad J. A. Shiddiky,

Matt Trau

Publication year - 2014

Publication title -

chemical communications

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.837

H-Index - 333

eISSN - 1364-548X

pISSN - 1359-7345

DOI - 10.1039/c3cc49607d

Subject(s) - biosensor , surface plasmon resonance , dna , dna methylation , inversion (geology) , chemistry , methylation , nanotechnology , materials science , biology , gene , biochemistry , nanoparticle , gene expression , paleontology , structural basin

DNA methylation has the potential to be a clinically important biomarker in cancer. This communication reports a real-time and label-free biosensing strategy for DNA methylation detection in the cancer cell line. This has been achieved by using surface plasmon resonance biosensing combined with the highly specific molecular inversion probe based amplification method, which requires only 50 ng of bisulfite treated genomic DNA.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research