Alkynyl monosaccharide analogues as a tool for evaluating Golgi glycosylation efficiency: application to Congenital Disorders of Glycosylation (CDG) | Zendy

Jorick Vanbeselaere | Zendy; Dorothée Vicogne | Zendy; Gert Matthijs | Zendy; Christophe Biot | Zendy; François Foulquier | Zendy; Yann Guérardel | Zendy

Open Access

Alkynyl monosaccharide analogues as a tool for evaluating Golgi glycosylation efficiency: application to Congenital Disorders of Glycosylation (CDG)

Author(s) -

Jorick Vanbeselaere,

Dorothée Vicogne,

Gert Matthijs,

Christophe Biot,

François Foulquier,

Yann Guérardel

Publication year - 2013

Publication title -

chemical communications

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.837

H-Index - 333

eISSN - 1364-548X

pISSN - 1359-7345

DOI - 10.1039/c3cc45914d

Subject(s) - glycosylation , golgi apparatus , glycan , sialic acid , monosaccharide , chemistry , labelling , biochemistry , computational biology , cell , biology , glycoprotein

The visualization of Golgi glycosylation defects in patients' cells with Congenital Disorders of Glycosylation (CDG) is challenging and necessitates the use of cumbersome glycan analysis methods that are barely adapted to clinical research. We show here that metabolic labelling of patient cells with alkyne-tagged sialic-acid (SiaNAl) enables an easy and reliable readout assay for the detection of CDG occurrence. It also provides valuable clues regarding the pathological processes by assessing the distribution of sialic acid analogues within the cells.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research