Open AccessAn artificial restriction DNA cutter for site-selective gene insertion in human cells
Author(s) -
Kenichiro Ito,
Narumi Shigi,
Makoto Komiyama
Publication year - 2013
Publication title -
chemical communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.837
H-Index - 333
eISSN - 1364-548X
pISSN - 1359-7345
DOI - 10.1039/c3cc43261k
Subject(s) - homologous recombination , dna , gene , restriction site , microbiology and biotechnology , genetics , reporter gene , chemistry , fluorescence , homologous chromosome , biology , restriction enzyme , gene expression , physics , quantum mechanics
With the use of a chemistry-based artificial restriction DNA cutter (combination of Ce(IV)-EDTA and a pair of pcPNA), both an antibiotic-resistance gene and a fluorescent reporter protein gene were incorporated into the targeted site through homologous recombination in human cells.
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