Molecular engineering of chromophores for combined second-harmonic and two-photon fluorescence in cellular imaging | Zendy

Evelien De Meulenaere | Zendy; Weiqiang Chen | Zendy; Stijn Van Cleuvenbergen | Zendy; MeiLing Zheng | Zendy; Sotiris Psilodimitrakopoulos | Zendy; Rik Paesen | Zendy; JeanMarc Taymans | Zendy; Marcel Ameloot | Zendy; Jos Vanderleyden | Zendy; Pablo LozaÁlvarez | Zendy; XuanMing Duan | Zendy; Koen Clays | Zendy

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Molecular engineering of chromophores for combined second-harmonic and two-photon fluorescence in cellular imaging

Author(s) -

Evelien De Meulenaere,

Weiqiang Chen,

Stijn Van Cleuvenbergen,

MeiLing Zheng,

Sotiris Psilodimitrakopoulos,

Rik Paesen,

JeanMarc Taymans,

Marcel Ameloot,

Jos Vanderleyden,

Pablo LozaÁlvarez,

XuanMing Duan,

Koen Clays

Publication year - 2012

Publication title -

chemical science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.687

H-Index - 172

eISSN - 2041-6539

pISSN - 2041-6520

DOI - 10.1039/c2sc00771a

Subject(s) - chromophore , carbazole , fluorescence , pyridinium , photochemistry , fluorescence lifetime imaging microscopy , second harmonic generation , two photon excitation microscopy , fluorescence microscope , materials science , chemistry , optics , physics , laser , organic chemistry

A series of chromophores with enhanced second- and third-order nonlinear optical properties were engineered for use in combined second-harmonic and two-photon fluorescence microscopy. Electron-accepting moieties imparted nonlinear optical properties to the chromophores. The electron-rich carbazole core served as a template towards one- or two-dimensional chromophores. More efficient acceptor groups (pyridinium, benzazolium, benzothiazolium) on the carbazole donor core resulted in improved second- and third-order nonlinear optical properties. A selection of these chromophores was tested in a cellular environment with a multimodal multiphoton microscope. The structural differences of the chromophores resulted in high selectivity for mitochondria or the nucleus in two-photon fluorescence and ranging from no signal to high selectivity for mitochondria in the SHG channel.

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