Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2′-modified nucleotides | Zendy

Vanessa Siegmund | Zendy; Tobias Santner | Zendy; Ronald Micura | Zendy; Andreas Marx | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2′-modified nucleotides

Author(s) -

Vanessa Siegmund,

Tobias Santner,

Ronald Micura,

Andreas Marx

Publication year - 2012

Publication title -

chemical communications

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.837

H-Index - 333

eISSN - 1364-548X

pISSN - 1359-7345

DOI - 10.1039/c2cc35028a

Subject(s) - t7 rna polymerase , nucleotide , polymerase , mutant , microbiology and biotechnology , rna polymerase , rna dependent rna polymerase , rna polymerase iii , rna , chemistry , biology , biochemistry , dna , gene , escherichia coli , bacteriophage

We present a screening assay based on fluorescence readout for the directed evolution of T7 RNA polymerase variants with acceptance of 2'-modified nucleotides. By using this screening we were able to identify a T7 RNA polymerase mutant with increased acceptance of 2'-methylseleno-2'-deoxyuridine 5'-triphosphate.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research