A self assembled monolayer based microfluidic sensor for urea detection | Zendy

Saurabh Srivastava | Zendy; Pratima R. Solanki | Zendy; Ajeet Kaushik | Zendy; Md. Azahar Ali | Zendy; Anchal Srivastava | Zendy; Bansi D. Malhotra | Zendy

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A self assembled monolayer based microfluidic sensor for urea detection

Author(s) -

Saurabh Srivastava,

Pratima R. Solanki,

Ajeet Kaushik,

Md. Azahar Ali,

Anchal Srivastava,

Bansi D. Malhotra

Publication year - 2011

Publication title -

nanoscale

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.038

H-Index - 224

eISSN - 2040-3372

pISSN - 2040-3364

DOI - 10.1039/c1nr10240k

Subject(s) - detection limit , cyclic voltammetry , fourier transform infrared spectroscopy , monolayer , urea , urease , glutamate dehydrogenase , analytical chemistry (journal) , self assembled monolayer , electrode , materials science , electrochemistry , nuclear chemistry , contact angle , chemistry , nanotechnology , chemical engineering , chromatography , organic chemistry , biochemistry , composite material , glutamate receptor , receptor , engineering

Urease (Urs) and glutamate dehydrogenase (GLDH) have been covalently co-immobilized onto a self-assembled monolayer (SAM) comprising of 10-carboxy-1-decanthiol (CDT) via EDC-NHS chemistry deposited onto one of the two patterned gold (Au) electrodes for estimation of urea using poly(dimethylsiloxane) based microfluidic channels (2 cm × 200 μm × 200 μm). The CDT/Au and Urs-GLDH/CDT/Au electrodes have been characterized using Fourier transform infrared (FTIR) spectroscopy, contact angle (CA), atomic force microscopy (AFM) and electrochemical cyclic voltammetry (CV) techniques. The electrochemical response measurement of a Urs-GLDH/CDT/Au bioelectrode obtained as a function of urea concentration using CV yield linearity as 10 to 100 mg dl(-1), detection limit as 9 mg dl(-1) and high sensitivity as 7.5 μA mM(-1) cm(-2).

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