Quantification of the orientations of pyrrolidine-based oxypeptide nucleic acid–DNA hybrid duplexes | Zendy

Mizuki Kitamatsu | Zendy; Masahiko Sisido | Zendy

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Quantification of the orientations of pyrrolidine-based oxypeptide nucleic acid–DNA hybrid duplexes

Author(s) -

Mizuki Kitamatsu,

Masahiko Sisido

Publication year - 2011

Publication title -

medchemcomm

Language(s) - English

Resource type - Journals

eISSN - 2040-2511

pISSN - 2040-2503

DOI - 10.1039/c0md00223b

Subject(s) - antiparallel (mathematics) , pyrrolidine , bodipy , nucleic acid , guanine , dna , fluorescence , quenching (fluorescence) , chemistry , oligonucleotide , combinatorial chemistry , biochemistry , stereochemistry , nucleotide , gene , physics , quantum mechanics , magnetic field

We describe the fluorescence quenching-based quantification of complementary parallel and antiparallel hybrids of DNAs with pyrrolidine-based oxypeptide nucleic acids (POPNAs). When BODIPY-modified DNAs as fluorescent probe formed hybrids with complementary POPNAs, fluorescence of the BODIPY was effectively quenched by the guanine unit and the Lys unit on the POPNAs. The orientations of hybrids of POPNA with DNA were estimated by the quenching efficiencies of two BODIPY-modified DNAs . As a result, we clarified that configurations of POPNAs affect the extent of orientation of the hybrid duplexes

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