Enzyme switch by complementary polymer pair system (CPPS) | Zendy

Shunsuke Tomita | Zendy; Len Ito | Zendy; Hiroshi Yamaguchi | Zendy; Genichi Konishi | Zendy; Yukio Nagasaki | Zendy; Kentaro Shiraki | Zendy

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Enzyme switch by complementary polymer pair system (CPPS)

Author(s) -

Shunsuke Tomita,

Len Ito,

Hiroshi Yamaguchi,

Genichi Konishi,

Yukio Nagasaki,

Kentaro Shiraki

Publication year - 2010

Publication title -

soft matter

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 170

eISSN - 1744-6848

pISSN - 1744-683X

DOI - 10.1039/c000930j

Subject(s) - enzyme , allylamine , ribonuclease , lysozyme , chemistry , polymer , amylase , acrylic acid , rnase p , biochemistry , cellulase , enzyme assay , combinatorial chemistry , polyelectrolyte , organic chemistry , rna , copolymer , gene

Manipulation of enzyme activity at will presents various research and industrial applications. Herein we describe development of a technology for inactivation and reactivation of enzyme activities using a polyanionic poly(acrylic acid) (PAAc) and a polycationic poly(allylamine) (PAA). Enzyme activities of ribonuclease A (RNase A), lysozyme, cellulase, and α-amylase were lost through addition of PAAc or PAA because of their binding to the enzymes. The activity of these enzymes except for α-amylase was then fully restored from the complex by the addition of oppositely charged polymers. Such manipulation of enzyme activity using a complementary polymer pair system (CPPS) enables the expansion of biomedical and biotechnological applications of the enzymes, including realization of protein delivery and intelligent bioreactors

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