Preparation, characterization and application of polyaniline nanospheres to biosensing | Zendy

Chetna Dhand | Zendy; Maumita Das | Zendy; Gajjala Sumana | Zendy; Avanish Kumar Srivastava | Zendy; Manoj Kumar Pandey | Zendy; CheolGi Kim | Zendy; Monika Datta | Zendy; Bansi D. Malhotra | Zendy

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Preparation, characterization and application of polyaniline nanospheres to biosensing

Author(s) -

Chetna Dhand,

Maumita Das,

Gajjala Sumana,

Avanish Kumar Srivastava,

Manoj Kumar Pandey,

CheolGi Kim,

Monika Datta,

Bansi D. Malhotra

Publication year - 2010

Publication title -

nanoscale

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.038

H-Index - 224

eISSN - 2040-3372

pISSN - 2040-3364

DOI - 10.1039/b9nr00346k

Subject(s) - polyaniline , materials science , cholesterol oxidase , ethylene glycol , scanning electron microscope , nuclear chemistry , fourier transform infrared spectroscopy , chemical engineering , transmission electron microscopy , nanocomposite , polymer chemistry , analytical chemistry (journal) , nanotechnology , polymerization , polymer , chemistry , organic chemistry , composite material , engineering , enzyme

Polyaniline nanospheres (PANI-NS) prepared by morphological transformation of micelle polymerized camphorsulfonic acid (CSA) doped polyaniline nanotubes (PANI-NT) in the presence of ethylene glycol (EG) have been characterized by X-ray diffraction, atomic force microscopy, transmission electron microscopy, scanning electron microscopy, Fourier transform infra-red and UV-Visible spectroscopy. A PANI-NS (60-80 nm) film deposited onto an indium-tin-oxide (ITO) coated glass plate by the solution casting method has been utilized for covalent immobilization of biomolecules (cholesterol oxidase (ChOx)) viaN-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry for fabrication of a cholesterol biosensor. The ChOx/PANI-NS/ITO bioelectrode detects cholesterol in the concentration range of 25 to 500 mg dL(-1) with sensitivity of 1.3 x 10(-3) mA mg(-1) dL and regression coefficient of 0.98. Further, this PANI-NS based bioelectrode shows fast response time (10 s), low Michaelis-Menten constant (2.5 mM) and shelf-life of 12 weeks. The spherical nanostructure observed in the final morphology of the PANI-NS film is attributed to hydrogen bonding interactions between PANI-NT and EG.

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