A microfluidic electroporation device for cell lysis | Zendy

Hang Lu | Zendy; Martin A. Schmidt | Zendy; Klavs F. Jensen | Zendy

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A microfluidic electroporation device for cell lysis

Author(s) -

Hang Lu,

Martin A. Schmidt,

Klavs F. Jensen

Publication year - 2004

Publication title -

lab on a chip

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.064

H-Index - 210

eISSN - 1473-0197

pISSN - 1473-0189

DOI - 10.1039/b406205a

Subject(s) - electroporation , lysis , microfluidics , miniaturization , membrane , nanotechnology , photolithography , materials science , organelle , nanobiotechnology , cell , chemistry , nanoparticle , biochemistry , gene

We demonstrate a micro-electroporation device for cell lysis prior to subcellular analysis. Simple circuit models show that electrical lysis method is advantageous because it is selective towards plasma membrane while leaving organelle membrane undamaged. In addition, miniaturization of this concept leads to negligible heat generation and bubble formation. The designed microdevices were fabricated using a combination of photolithography, metal-film deposition, and electroplating. We demonstrate the electro-lysis of human carcinoma cells in these devices to release the subcellular materials.

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