Generation of Hprt-disrupted rat through mouse←rat ES chimeras | Zendy

Ayako Isotani | Zendy; Kazuo Yamagata | Zendy; Masaru Okabe | Zendy; Masahito Ikawa | Zendy

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Generation of Hprt-disrupted rat through mouse←rat ES chimeras

Author(s) -

Ayako Isotani,

Kazuo Yamagata,

Masaru Okabe,

Masahito Ikawa

Publication year - 2016

Publication title -

scientific reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.24

H-Index - 213

ISSN - 2045-2322

DOI - 10.1038/srep24215

Subject(s) - chimera (genetics) , hypoxanthine guanine phosphoribosyltransferase , biology , microbiology and biotechnology , computational biology , genetics , gene , mutant

We established rat embryonic stem (ES) cell lines from a double transgenic rat line which harbours CAG-GFP for ubiquitous expression of GFP in somatic cells and Acr3-EGFP for expression in sperm (green body and green sperm: GBGS rat). By injecting the GBGS rat ES cells into mouse blastocysts and transplanting them into pseudopregnant mice, rat spermatozoa were produced in mouse←rat ES chimeras. Rat spermatozoa from the chimeric testis were able to fertilize eggs by testicular sperm extraction combined with intracytoplasmic sperm injection (TESE-ICSI). In the present paper, we disrupted rat hypoxanthine-guanine phosphoribosyl transferase ( Hprt ) gene in ES cells and produced a Hprt -disrupted rat line using the mouse←rat ES chimera system. The mouse←rat ES chimera system demonstrated the dual advantages of space conservation and a clear indication of germ line transmission in knockout rat production.

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