Paeonol and danshensu combination attenuates apoptosis in myocardial infarcted rats by inhibiting oxidative stress: Roles of Nrf2/HO-1 and PI3K/Akt pathway | Zendy

Hua Li | Zendy; Fan Song | Zendy; Linrui Duan | Zendy; JuanJuan Sheng | Zendy; Yanhua Xie | Zendy; Qian Yang | Zendy; Ying Chen | Zendy; Qianqian Dong | Zendy; BangLe Zhang | Zendy; Siwang Wang | Zendy

Open Access

Paeonol and danshensu combination attenuates apoptosis in myocardial infarcted rats by inhibiting oxidative stress: Roles of Nrf2/HO-1 and PI3K/Akt pathway

Author(s) -

Hua Li,

Fan Song,

Linrui Duan,

JuanJuan Sheng,

Yanhua Xie,

Qian Yang,

Ying Chen,

Qianqian Dong,

BangLe Zhang,

Siwang Wang

Publication year - 2016

Publication title -

scientific reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.24

H-Index - 213

ISSN - 2045-2322

DOI - 10.1038/srep23693

Subject(s) - paeonol , pi3k/akt/mtor pathway , protein kinase b , oxidative stress , pharmacology , apoptosis , tunel assay , cardioprotection , myocardial infarction , medicine , signal transduction , chemistry , biochemistry , pathology , alternative medicine

Paeonol and danshensu is the representative active ingredient of traditional Chinese medicinal herbs Cortex Moutan and Radix Salviae Milthiorrhizae, respectively. Paeonol and danshensu combination (PDSS) has putative cardioprotective effects in treating ischemic heart disease (IHD). However, the evidence for the protective effect is scarce and the pharmacological mechanisms of the combination remain unclear. The present study was designed to investigate the protective effect of PDSS on isoproterenol (ISO)-induced myocardial infarction in rats and to elucidate the potential mechanism. Assays of creatine kinase-MB, cardiac troponin I and T and histopathological analysis revealed PDSS significantly prevented myocardial injury induced by ISO. The ISO-induced profound elevation of oxidative stress was also suppressed by PDSS. TUNEL and caspase-3 activity assay showed that PDSS significantly inhibited apoptosis in myocardia. In exploring the underlying mechanisms of PDSS, we found PDSS enhanced the nuclear translocation of Nrf2 in myocardial injured rats. Furthermore, PDSS increased phosphorylated PI3K and Akt, which may in turn activate antioxidative and antiapoptotic signaling events in rat. These present findings demonstrated that PDSS exerts significant cardioprotective effects against ISO-induced myocardial infarction in rats. The protective effect is, at least partly, via activation of Nrf2/HO-1 signaling and involvement of the PI3K/Akt cell survival signaling pathway.

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