Simultaneous determination of 8-oxo-2’-deoxyguanosine and 8-oxo-2’-deoxyadenosine in human retinal DNA by liquid chromatography nanoelectrospray-tandem mass spectrometry | Zendy

Bin Ma | Zendy; Jing Meng | Zendy; Peter W. Villalta | Zendy; Rebecca J. Kapphahn | Zendy; Sandra R. Montezuma | Zendy; Deborah A. Ferrington | Zendy; Irina Stepanov | Zendy

Open Access

Simultaneous determination of 8-oxo-2’-deoxyguanosine and 8-oxo-2’-deoxyadenosine in human retinal DNA by liquid chromatography nanoelectrospray-tandem mass spectrometry

Author(s) -

Bin Ma,

Jing Meng,

Peter W. Villalta,

Rebecca J. Kapphahn,

Sandra R. Montezuma,

Deborah A. Ferrington,

Irina Stepanov

Publication year - 2016

Publication title -

scientific reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.24

H-Index - 213

ISSN - 2045-2322

DOI - 10.1038/srep22375

Subject(s) - tandem mass spectrometry , chemistry , dna , retinal , mitochondrial dna , deoxyguanosine , adduct , deoxyadenosine , retinal pigment epithelium , dna damage , oxidative phosphorylation , macular degeneration , mass spectrometry , liquid chromatography–mass spectrometry , microbiology and biotechnology , chromatography , biochemistry , biology , ophthalmology , medicine , organic chemistry , gene

Age-related macular degeneration (AMD) is the leading cause of blindness among older adults in the developed world. Oxidative damage to mitochondrial DNA (mtDNA) in the retinal pigment epithelium (RPE) may play a key role in AMD. Measurement of oxidative DNA lesions such as 8-oxo-2’-deoxyguanosine (8-oxo-dG) and 8-oxo-2’-deoxyadenosine (8-oxo-dA) in diseased RPE could provide important insights into the mechanism of AMD development. We have developed a liquid chromatography-nanoelectrospray ionization-tandem mass spectrometry method for simultaneous analysis of 8-oxo-dG and 8-oxo-dA in human retinal DNA. The developed method was applied to the analysis of retinal DNA from 5 donors with AMD and 5 control donors without AMD. In mtDNA, the levels of 8-oxo-dG in controls and AMD donors averaged 170 and 188, and 8-oxo-dA averaged 11 and 17 adducts per 10 6 bases, respectively. In nuclear DNA, the levels of 8-oxo-dG in controls and AMD donors averaged 0.54 and 0.96, and 8-oxo-dA averaged 0.04 and 0.05 adducts per 10 6 bases, respectively. This highly sensitive method allows for the measurement of both adducts in very small amounts of DNA and can be used in future studies investigating the pathophysiological role of 8-oxo-dG and 8-oxo-dA in AMD and other oxidative damage-related diseases in humans.

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