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Mesenchymal stem cells (MSCs) are multipotent cells, which can give rise to variety of cell types, including adipocytes and osteoblasts. Previously, we have shown that cysteine dioxygenase type 1 ( Cdo1 ) promoted adipogenesis of primary mouse bone marrow stromal cells (BMSCs) and 3T3-L1 pre-adipocytes via interaction with Pparγ. However, the role of  Cdo1  in osteogenesis remains unclear. Here, we demonstrated that expression of  Cdo1  was elevated during osteoblastic differentiation of BMSCs  in vitro . Interestingly, knockdown of  Cdo1  by siRNA led to an increased expression of osteogenic related genes, elevated alkaline phosphatase (ALP) activity, and enhanced mineralization. Overexpression of  Cdo1  in BMSCs inversely suppressed the osteogenesis. Furthermore, we found that overexpression of  Cdo1  impaired Wnt signaling and restricted the Wnt3a induced expression of osteogenic transcriptional factors, such as  Runx2  and  Dlx5 . Collectively, our findings indicate  Cdo1  suppresses osteogenic differentiation of BMSCs, through a potential mechanism which involves in Wnt signaling reduction concomitantly.

Cysteine Dioxygenase Type 1 Inhibits Osteogenesis by Regulating Wnt Signaling in Primary Mouse Bone Marrow Stromal Cells