GADD34 Gene Restores Virulence in Viral Vector Used in Experimental Stroke Study | Zendy

Christopher McCabe | Zendy; F. E. White | Zendy; Stuart M. Brown | Zendy; I. Mhairi Macrae | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

GADD34 Gene Restores Virulence in Viral Vector Used in Experimental Stroke Study

Author(s) -

Christopher McCabe,

F. E. White,

Stuart M. Brown,

I. Mhairi Macrae

Publication year - 2007

Publication title -

journal of cerebral blood flow and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.167

H-Index - 193

eISSN - 1559-7016

pISSN - 0271-678X

DOI - 10.1038/sj.jcbfm.9600565

Subject(s) - virulence , stroke (engine) , vector (molecular biology) , viral vector , virology , medicine , gene , biology , neuroscience , genetics , physics , recombinant dna , thermodynamics

GADD34 is expressed in the ischaemic brain and reverses protein synthesis shutdown. Consequently, GADD34 could have neuroprotective potential in stroke. BHK medium, a replication-deficient HSV viral vector (HSV1716) with no insert or containing full-length GADD34, the N terminal or a conserved portion of the gene, was injected into mouse brain before stroke. Infarct size was 1.0+/-0.26, 1.19+/-0.36, 1.5+/-0.36, 1.3+/-0.36, and 1.1+/-0.28 mm3, respectively. The increase in infarct size with full-length GADD34 was statistically significant (P<0.05). Immunohistochemistry confirmed viral protein expression. Tissue culture studies revealed GADD34 gene restored virulence in HSV1716, suggesting that HSV virulence, rather than increased GADD34, exacerbated ischaemic damage.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research