Open AccessYeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
Author(s) -
Vikram Virdi,
Jorge Palací,
Bram Laukens,
Stefan Ryckaert,
Eric Cox,
Erik Vanderbeke,
Anna Depicker,
Nico Callewaert
Publication year - 2019
Publication title -
nature biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 15.358
H-Index - 445
eISSN - 1546-1696
pISSN - 1087-0156
DOI - 10.1038/s41587-019-0070-x
Subject(s) - antibody , pichia pastoris , yeast , microbiology and biotechnology , biology , enterotoxigenic escherichia coli , immunoglobulin a , escherichia coli , immunoglobulin e , pichia , immunoglobulin g , immunology , gene , recombinant dna , biochemistry , enterotoxin
Oral antibodies that interfere with gastrointestinal targets and can be manufactured at scale are needed. Here we show that a single-gene-encoded monomeric immunoglobulin A (IgA)-like antibody, composed of camelid variable single domain antibodies (VHH) fused to IgA Fc (mVHH-IgA), prevents infection by enterotoxigenic Escherichia coli (F4-ETEC) in piglets. The mVHH-IgA can be produced in soybean seeds or secreted from the yeast Pichia pastoris, freeze- or spray-dried and orally delivered within food.