Open AccessCo-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish
Author(s) -
Dm M. Langenau,
Matthew D. Keefe,
Ny Y. Storer,
Ca A. Jette,
Ach C. H. Smith,
Cj J. Ceol,
Caitlin Bourque,
A. Thomas Look,
Li I. Zon
Publication year - 2008
Publication title -
oncogene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.395
H-Index - 342
eISSN - 1476-5594
pISSN - 0950-9232
DOI - 10.1038/onc.2008.56
Subject(s) - biology , zebrafish , transgene , genetically modified mouse , carcinogenesis , gene , microinjection , cancer research , cancer , computational biology , microbiology and biotechnology , genetics
The zebrafish has emerged as a powerful genetic model of cancer, but has been limited by the use of stable transgenic approaches to induce disease. Here, a co-injection strategy is described that capitalizes on both the numbers of embryos that can be microinjected and the ability of transgenes to segregate together and exert synergistic effects in forming tumors. Using this mosaic transgenic approach, gene pathways involved in tumor initiation and radiation sensitivity have been identified.