Open AccessThe Tetrahymena telomerase p75–p45–p19 subcomplex is a unique CST complex
Author(s) -
Bingbing Wan,
Ting Tang,
Heather E. Upton,
Shuai Jin,
Yuanze Zhou,
Li Song,
Juan Chen,
J.S. Brunzelle,
Zhixiong Zeng,
Kathleen Collins,
Jian Wu,
Ming Lei
Publication year - 2015
Publication title -
nature structural and molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.448
H-Index - 270
eISSN - 1545-9993
pISSN - 1545-9985
DOI - 10.1038/nsmb.3126
Subject(s) - tetrahymena , telomerase , telomere , microbiology and biotechnology , protein subunit , dna , biology , chemistry , biochemistry , gene
Tetrahymena telomerase holoenzyme subunits p75, p45 and p19 form a subcomplex (7-4-1) peripheral to the catalytic core. We report structures of p45 and p19 and reveal them as the Stn1 and Ten1 subunits of the CST complex, which stimulates telomerase complementary-strand synthesis. 7-4-1 binds telomeric single-stranded DNA, and mutant p19 overexpression causes telomere 3'-overhang elongation. We propose that telomerase-tethered Tetrahymena CST coordinates telomere G-strand and C-strand synthesis.
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