Open AccessStructural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation
Author(s) -
Martin Jinek,
Marc R. Fabian,
Scott M. Coyle,
Nahum Sonenberg,
Jennifer A. Doudna
Publication year - 2010
Publication title -
nature structural and molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.448
H-Index - 270
eISSN - 1545-9993
pISSN - 1545-9985
DOI - 10.1038/nsmb.1768
Subject(s) - biology , gene silencing , microbiology and biotechnology , genetics , argonaute , psychological repression , microrna , rna binding protein , rna interference , rna , gene , gene expression
GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing.