Novel protein antigen (JHP940) from the genomic plasticity region of Helicobacter pylori induces TNF-alpha and Interleukin- 8 secretion by human macrophages | Zendy

Niyaz Ahmed | Zendy; Ayesha Alvi | Zendy; Mohammed Rizwan | Zendy

Open Access

Novel protein antigen (JHP940) from the genomic plasticity region of Helicobacter pylori induces TNF-alpha and Interleukin- 8 secretion by human macrophages

Author(s) -

Niyaz Ahmed,

Ayesha Alvi,

Mohammed Rizwan

Publication year - 2007

Publication title -

nature precedings

Language(s) - English

Resource type - Journals

ISSN - 1756-0357

DOI - 10.1038/npre.2007.864.1

Subject(s) - caga , helicobacter pylori , genotyping , locus (genetics) , biology , tumor necrosis factor alpha , secretion , gene , inflammation , chemokine , immunology , genetics , genotype , virulence , biochemistry

Plasticity region of the H. pylori genome comprises strain specific gene loci. We performed genotyping and functional biology of one such locus (jhp940) that was previously found to be functionally unknown but present in gastric cancer associated strains from many different countries. We found its geographic prevalence to be independent of cagA presence and disease status. Cloning, expression and purification of the JHP940 revealed a novel, ~36kDa protein in biologically active form. The same induced strong and significant levels of TNF-alpha and Interleukin-8 in human macrophages. Induction of these cytokines by JHP940 points to its putative role in chronic gastric inflammation and various other outcomes of H. pylori infection including gastric cancer

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