Open AccessSouthern Blotting and Related DNA Detection Techniques
Author(s) -
Terence A. Brown
Publication year - 2001
Publication title -
encyclopedia of life sciences
Language(s) - English
Resource type - Reports
DOI - 10.1038/npg.els.0000996
Subject(s) - nitrocellulose , blot , southern blot , dna , gel electrophoresis , microbiology and biotechnology , chemistry , membrane , chromatography , biology , biochemistry , gene
Southern blotting is a technique for transfer of DNA molecules from an electrophoresis gel to a nitrocellulose or nylon membrane, and is carried out prior to detection of specific molecules by hybridization probing. Keywords: southern blotting; DNA preparation; DNA/DNA hybridization; hybridization probing; restriction fragment length polymorphism (RFLP)
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