Highly parallel direct RNA sequencing on an array of nanopores | Zendy

Daniel R. Garalde | Zendy; Elizabeth A. Snell | Zendy; Daniel Jachimowicz | Zendy; Botond Sipos | Zendy; Joseph H Lloyd | Zendy; Mark Bruce | Zendy; Nadia Pantic | Zendy; Tigist Admassu | Zendy; Phillip James | Zendy; Anthony Warland | Zendy; Michael R. Jordan | Zendy; Jonah Ciccone | Zendy; Sabrina Serra | Zendy; Jemma Keenan | Zendy; Samuel Martin | Zendy; Luke A. McNeill | Zendy; E. Jayne Wallace | Zendy; Lakmal Jayasinghe | Zendy; Chris Wright | Zendy; Javier Blasco | Zendy; Stephen L. Young | Zendy; Denise Brocklebank | Zendy; Sissel Juul | Zendy; James Clarke | Zendy; Andrew J. Heron | Zendy; Daniel J. Turner | Zendy

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Highly parallel direct RNA sequencing on an array of nanopores

Author(s) -

Daniel R. Garalde,

Elizabeth A. Snell,

Daniel Jachimowicz,

Botond Sipos,

Joseph H Lloyd,

Mark Bruce,

Nadia Pantic,

Tigist Admassu,

Phillip James,

Anthony Warland,

Michael R. Jordan,

Jonah Ciccone,

Sabrina Serra,

Jemma Keenan,

Samuel Martin,

Luke A. McNeill,

E. Jayne Wallace,

Lakmal Jayasinghe,

Chris Wright,

Javier Blasco,

Stephen L. Young,

Denise Brocklebank,

Sissel Juul,

James Clarke,

Andrew J. Heron,

Daniel J. Turner

Publication year - 2018

Publication title -

nature methods

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 19.469

H-Index - 318

eISSN - 1548-7105

pISSN - 1548-7091

DOI - 10.1038/nmeth.4577

Subject(s) - rna , nanopore , nanopore sequencing , computational biology , rna splicing , biology , transcription (linguistics) , reverse transcriptase , genetics , dna sequencing , gene , nanotechnology , linguistics , philosophy , materials science

Sequencing the RNA in a biological sample can unlock a wealth of information, including the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional state of organisms. However, current methods have limitations due to short read lengths and reverse transcription or amplification biases. Here we demonstrate nanopore direct RNA-seq, a highly parallel, real-time, single-molecule method that circumvents reverse transcription or amplification steps. This method yields full-length, strand-specific RNA sequences and enables the direct detection of nucleotide analogs in RNA.

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