Highly efficient Cas9-mediated transcriptional programming | Zendy

Alejandro Chavez | Zendy; Jonathan Scheiman | Zendy; Suhani Vora | Zendy; Benjamin W. Pruitt | Zendy; Marcelle Tuttle | Zendy; Eswar Prasad R. Iyer | Zendy; Shuailiang Lin | Zendy; Samira Kiani | Zendy; Christopher D. Guzman | Zendy; Daniel J. Wiegand | Zendy; Dmitry TerOvanesyan | Zendy; Jonathan L. Braff | Zendy; Noah Davidsohn | Zendy; Benjamin E. Housden | Zendy; Norbert Perrimon | Zendy; Ron Weiss | Zendy; John Aach | Zendy; James J. Collins | Zendy; George M. Church | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Highly efficient Cas9-mediated transcriptional programming

Author(s) -

Alejandro Chavez,

Jonathan Scheiman,

Suhani Vora,

Benjamin W. Pruitt,

Marcelle Tuttle,

Eswar Prasad R. Iyer,

Shuailiang Lin,

Samira Kiani,

Christopher D. Guzman,

Daniel J. Wiegand,

Dmitry TerOvanesyan,

Jonathan L. Braff,

Noah Davidsohn,

Benjamin E. Housden,

Norbert Perrimon,

Ron Weiss,

John Aach,

James J. Collins,

George M. Church

Publication year - 2015

Publication title -

nature methods

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 19.469

H-Index - 318

eISSN - 1548-7105

pISSN - 1548-7091

DOI - 10.1038/nmeth.3312

Subject(s) - cas9 , nuclease , biology , activator (genetics) , induced pluripotent stem cell , gene , microbiology and biotechnology , regulator , transcription factor , crispr , computational biology , embryonic stem cell , genetics

The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research