Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange | Zendy

Yidan Ding | Zendy; Jiao Li | Zendy; Jhon R. Enterina | Zendy; Yi Shen | Zendy; Issan Zhang | Zendy; Paul Tewson | Zendy; Gary Mo | Zendy; Jin Zhang | Zendy; Anne Marie Quinn | Zendy; Thomas E. Hughes | Zendy; Dušica Maysinger | Zendy; Spencer C. Alford | Zendy; Yan Zhang | Zendy; Robert E. Campbell | Zendy

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Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

Author(s) -

Yidan Ding,

Jiao Li,

Jhon R. Enterina,

Yi Shen,

Issan Zhang,

Paul Tewson,

Gary Mo,

Jin Zhang,

Anne Marie Quinn,

Thomas E. Hughes,

Dušica Maysinger,

Spencer C. Alford,

Yan Zhang,

Robert E. Campbell

Publication year - 2015

Publication title -

nature methods

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 19.469

H-Index - 318

eISSN - 1548-7105

pISSN - 1548-7091

DOI - 10.1038/nmeth.3261

Subject(s) - biosensor , fluorescence , green fluorescent protein , intramolecular force , fluorescent protein , chemistry , biophysics , intermolecular force , förster resonance energy transfer , biochemistry , nanotechnology , microbiology and biotechnology , biology , molecule , gene , stereochemistry , materials science , physics , organic chemistry , quantum mechanics

We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities.

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