Tracking protein aggregation and mislocalization in cells with flow cytometry | Zendy

Yasmin M. Ramdzan | Zendy; Saskia Polling | Zendy; Cheryl Chia | Zendy; Ivan H. W. Ng | Zendy; Angelique R. Ormsby | Zendy; Nathan P. Croft | Zendy; Anthony W. Purcell | Zendy; Marie A. Bogoyevitch | Zendy; Dominic C.H. Ng | Zendy; Paul A. Gleeson | Zendy; Danny M. Hatters | Zendy

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Tracking protein aggregation and mislocalization in cells with flow cytometry

Author(s) -

Yasmin M. Ramdzan,

Saskia Polling,

Cheryl Chia,

Ivan H. W. Ng,

Angelique R. Ormsby,

Nathan P. Croft,

Anthony W. Purcell,

Marie A. Bogoyevitch,

Dominic C.H. Ng,

Paul A. Gleeson,

Danny M. Hatters

Publication year - 2012

Publication title -

nature methods

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 19.469

H-Index - 318

eISSN - 1548-7105

pISSN - 1548-7091

DOI - 10.1038/nmeth.1930

Subject(s) - flow cytometry , golgi apparatus , stress granule , cytoplasm , microbiology and biotechnology , protein aggregation , inclusion bodies , oligomer , granule (geology) , biophysics , chemistry , protein subcellular localization prediction , biology , biochemistry , recombinant dna , endoplasmic reticulum , paleontology , messenger rna , gene , organic chemistry , translation (biology)

We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington's disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.

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