A secreted luciferase for ex vivo monitoring of in vivo processes | Zendy

Thomas Würdinger | Zendy; Christian E. Badr | Zendy; Lisa Pike | Zendy; Ruben H. de Kleine | Zendy; Ralph Weissleder | Zendy; Xandra O. Breakefield | Zendy; Bakhos A. Tannous | Zendy

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A secreted luciferase for ex vivo monitoring of in vivo processes

Author(s) -

Thomas Würdinger,

Christian E. Badr,

Lisa Pike,

Ruben H. de Kleine,

Ralph Weissleder,

Xandra O. Breakefield,

Bakhos A. Tannous

Publication year - 2008

Publication title -

nature methods

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 19.469

H-Index - 318

eISSN - 1548-7105

pISSN - 1548-7091

DOI - 10.1038/nmeth.1177

Subject(s) - luciferase , bioluminescence , in vivo , luciferases , ex vivo , bioluminescence imaging , viability assay , microbiology and biotechnology , biology , chemistry , cell , biochemistry , transfection , gene

Luciferases are widely used to monitor biological processes. Here we describe the naturally secreted Gaussia princeps luciferase (Gluc) as a highly sensitive reporter for quantitative assessment of cells in vivo by measuring its concentration in blood. The Gluc blood assay complements in vivo bioluminescence imaging, which has the ability to localize the signal and provides a multifaceted assessment of cell viability, proliferation and location in experimental disease and therapy models.

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