Differentiation of human pluripotent stem cells to cells similar to cord-blood endothelial colony–forming cells | Zendy

Nutan Prasain | Zendy; Man Ryul Lee | Zendy; Sasidhar Vemula | Zendy; J. Luke Meador | Zendy; Momoko Yoshimoto | Zendy; Michael J. Ferkowicz | Zendy; Alexa Fett | Zendy; Manav Gupta | Zendy; Brian M. Rapp | Zendy; M. Reza Saadatzadeh | Zendy; Michael Ginsberg | Zendy; Olivier Elemento | Zendy; Younghee Lee | Zendy; Sherry L. VoytikHarbin | Zendy; HyungMin Chung | Zendy; Ki Sung Hong | Zendy; Emma Reid | Zendy; Christina O’Neill | Zendy; Reinhold J. Medina | Zendy; Alan W. Stitt | Zendy; Michael P. Murphy | Zendy; Shahin Rafii | Zendy; Hal E. Broxmeyer | Zendy; Mervin C. Yöder | Zendy

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Differentiation of human pluripotent stem cells to cells similar to cord-blood endothelial colony–forming cells

Author(s) -

Nutan Prasain,

Man Ryul Lee,

Sasidhar Vemula,

J. Luke Meador,

Momoko Yoshimoto,

Michael J. Ferkowicz,

Alexa Fett,

Manav Gupta,

Brian M. Rapp,

M. Reza Saadatzadeh,

Michael Ginsberg,

Olivier Elemento,

Younghee Lee,

Sherry L. VoytikHarbin,

HyungMin Chung,

Ki Sung Hong,

Emma Reid,

Christina O’Neill,

Reinhold J. Medina,

Alan W. Stitt,

Michael P. Murphy,

Shahin Rafii,

Hal E. Broxmeyer,

Mervin C. Yöder

Publication year - 2014

Publication title -

nature biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 15.358

H-Index - 445

eISSN - 1546-1696

pISSN - 1087-0156

DOI - 10.1038/nbt.3048

Subject(s) - induced pluripotent stem cell , microbiology and biotechnology , stem cell , endothelial stem cell , embryonic stem cell , cord blood , biology , kosr , adult stem cell , induced stem cells , immunology , in vitro , genetics , gene

The ability to differentiate human pluripotent stem cells into endothelial cells with properties of cord-blood endothelial colony-forming cells (CB-ECFCs) may enable the derivation of clinically relevant numbers of highly proliferative blood vessel-forming cells to restore endothelial function in patients with vascular disease. We describe a protocol to convert human induced pluripotent stem cells (hiPSCs) or embryonic stem cells (hESCs) into cells similar to CB-ECFCs at an efficiency of >10(8) ECFCs produced from each starting pluripotent stem cell. The CB-ECFC-like cells display a stable endothelial phenotype with high clonal proliferative potential and the capacity to form human vessels in mice and to repair the ischemic mouse retina and limb, and they lack teratoma formation potential. We identify Neuropilin-1 (NRP-1)-mediated activation of KDR signaling through VEGF165 as a critical mechanism for the emergence and maintenance of CB-ECFC-like cells.

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