CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells | Zendy

Xuelian Wang | Zendy; Xiumin Huang | Zendy; Xiuli Fang | Zendy; Youzhong Zhang | Zendy; Wanpeng Wang | Zendy

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CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells

Author(s) -

Xuelian Wang,

Xiumin Huang,

Xiuli Fang,

Youzhong Zhang,

Wanpeng Wang

Publication year - 2016

Publication title -

molecular therapy — nucleic acids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.208

H-Index - 59

ISSN - 2162-2531

DOI - 10.1038/mtna.2016.95

Subject(s) - crispr , genome engineering , cas9 , genome editing , computational biology , human genome , genome , computer science , biology , software engineering , engineering , genetics , gene

Targeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms. They can be notably used to facilitate effective genome manipulation in eukaryotic cells by clearly detailing a 20-nt targeting sequence inside its directed RNA. We discuss the most recent advancements in the molecular basis of the type II CRISPR/Cas system and encapsulate applications and elements affecting its use in human cells. We also propose possible applications covering its uses ranging from basic science to implementation in the clinic

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