Open AccessIn Vivo Selection Against Human Colorectal Cancer Xenografts Identifies an Aptamer That Targets RNA Helicase Protein DHX9
Author(s) -
Jing Mi,
Partha Ray,
Jenny Liu,
ChienTsun Kuan,
Jennifer Xu,
David S. Hsu,
Bruce A. Sullenger,
Rebekah R. White,
Bryan M. Clary
Publication year - 2016
Publication title -
molecular therapy — nucleic acids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.208
H-Index - 59
ISSN - 2162-2531
DOI - 10.1038/mtna.2016.27
Subject(s) - aptamer , in vivo , rna , rna helicase a , helicase , biology , cancer , colorectal cancer , cancer research , computational biology , microbiology and biotechnology , genetics , gene
The ability to selectively target disease-related tissues with molecules is critical to the design of effective therapeutic and diagnostic reagents. Recognizing the differences between the in vivo environment and in vitro conditions, we employed an in vivo selection strategy to identify RNA aptamers (targeting motifs) that could localize to tumor in situ. One of the selected molecules is an aptamer that binds to the protein DHX9, an RNA helicase that is known to be upregulated in colorectal cancer. Upon systemic administration, the aptamer preferentially localized to the nucleus of cancer cells in vivo and thus has the potential to be used for targeted delivery
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