Immunohistochemical Detection of Hepatocellular Carcinoma in the Setting of Ongoing Necrosis after Radiofrequency Ablation | Zendy

Tomoo Itoh | Zendy; Yasuko Orba | Zendy; Hidehiro Takei | Zendy; Yusuke Ishida | Zendy; Makoto Saitoh | Zendy; Hideaki Nakamura | Zendy; Takashi Meguro | Zendy; Shoichi Horita | Zendy; Miri Fujita | Zendy; Kazuo Nagashima | Zendy

Open Access

Immunohistochemical Detection of Hepatocellular Carcinoma in the Setting of Ongoing Necrosis after Radiofrequency Ablation

Author(s) -

Tomoo Itoh,

Yasuko Orba,

Hidehiro Takei,

Yusuke Ishida,

Makoto Saitoh,

Hideaki Nakamura,

Takashi Meguro,

Shoichi Horita,

Miri Fujita,

Kazuo Nagashima

Publication year - 2002

Publication title -

modern pathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.596

H-Index - 153

eISSN - 1530-0285

pISSN - 0893-3952

DOI - 10.1038/modpathol.3880501

Subject(s) - necrosis , pathology , cytoplasm , immunohistochemistry , hepatocellular carcinoma , cancer cell , radiofrequency ablation , biology , antibody , cancer , medicine , cancer research , ablation , microbiology and biotechnology , immunology , genetics

After radiofrequency ablation (RFA), hepatocellular carcinoma undergoes complete necrosis and an ongoing necrosis that is irreversible and characterized histologically by disrupted cell outlines, homogenous cytoplasmic eosinophilia, and preserved nuclear staining, with the cells appearing quite distinct from viable cancer cells. Antibody to detect single-stranded DNA (ssDNA) specifically labeled nuclei in the setting of ongoing necrosis, but not viable tumor cells, whereas human mitochondrial antibody labeled the cytoplasm of viable cells but not cells of ongoing necrosis. The results demonstrate that RFA causes denaturation of both DNA and proteins and that the immunohistochemistry of ssDNA and mitochondrial protein is useful in detection of ongoing necrosis after RFA and provides pathological information on the validity of this procedure.

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