Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization—a correlative study with urine PCA3 and TMPRSS2-ERG | Zendy

Joshua I. Warrick | Zendy; Scott A. Tomlins | Zendy; Shan L. Carskadon | Zendy; Allison M Young | Zendy; Javed Siddiqui | Zendy; John T. Wei | Zendy; Arul M. Chinnaiyan | Zendy; Lakshmi P. Kunju | Zendy; Nallasivam Palanisamy | Zendy

Open Access

Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization—a correlative study with urine PCA3 and TMPRSS2-ERG

Author(s) -

Joshua I. Warrick,

Scott A. Tomlins,

Shan L. Carskadon,

Allison M Young,

Javed Siddiqui,

John T. Wei,

Arul M. Chinnaiyan,

Lakshmi P. Kunju,

Nallasivam Palanisamy

Publication year - 2013

Publication title -

modern pathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.596

H-Index - 153

eISSN - 1530-0285

pISSN - 0893-3952

DOI - 10.1038/modpathol.2013.169

Subject(s) - pca3 , prostate cancer , tmprss2 , prostate , intraepithelial neoplasia , medicine , prostatectomy , cancer , erg , in situ hybridization , pathology , biology , messenger rna , disease , retinal , covid-19 , infectious disease (medical specialty) , ophthalmology , biochemistry , gene

PCA3 is a prostate-specific non-coding RNA, with utility as a urine-based early detection biomarker. Here, we report the evaluation of tissue PCA3 expression by RNA in situ hybridization in a cohort of 41 mapped prostatectomy specimens. We compared tissue PCA3 expression with tissue level ERG expression and matched pre-prostatectomy urine PCA3 and TMPRSS2-ERG levels. Across 136 slides containing 138 foci of prostate cancer, PCA3 was expressed in 55% of cancer foci and 71% of high-grade prostatic intraepithelial neoplasia foci. Overall, the specificity of tissue PCA3 was >90% for prostate cancer and high-grade prostatic intraepithelial neoplasia combined. Tissue PCA3 cancer expression was not significantly associated with urine PCA3 expression. PCA3 and ERG positivity in cancer foci was positively associated (P<0.01). We report the first comprehensive assessment of PCA3 expression in prostatectomy specimens, and find limited correlation between tissue PCA3 and matched urine in prostate cancer.

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