Open AccessImmunophenotyping of acute leukemia and lymphoproliferative disorders: a consensus proposal of the European LeukemiaNet Work Package 10
Author(s) -
Marie C. Béné,
Thomas Nebe,
Peter Bettelheim,
Barbara Buldini,
Horia Bumbea,
Wolfgang Kern,
Francis Lacombe,
P Lemež,
Iuri Marinov,
Estella Matutes,
Marc Maynadié,
Uta Oelschlägel,
Alberto Órfão,
Richard Schabath,
Max Solenthaler,
G. Tschurtschenthaler,
Ana-Maria Vlădăreanu,
Gina Zini,
G Fauré,
Anna Porwit
Publication year - 2011
Publication title -
leukemia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.539
H-Index - 192
eISSN - 1476-5551
pISSN - 0887-6924
DOI - 10.1038/leu.2010.312
Subject(s) - immunophenotyping , medicine , pathology , immunology , flow cytometry
The European LeukemiaNet (ELN), workpackage 10 (WP10) was designed to deal with diagnosis matters using morphology and immunophenotyping. This group aimed at establishing a consensus on the required reagents for proper immunophenotyping of acute leukemia and lymphoproliferative disorders. Animated discussions within WP10, together with the application of the Delphi method of proposals circulation, quickly led to post-consensual immunophenotyping panels for disorders on the ELN website. In this report, we established a comprehensive description of these panels, both mandatory and complementary, for both types of clinical conditions. The reason for using each marker, sustained by relevant literature information, is provided in detail. With the constant development of immunophenotyping techniques in flow cytometry and related software, this work aims at providing useful guidelines to perform the most pertinent exploration at diagnosis and for follow-up, with the best cost benefit in diseases, the treatment of which has a strong impact on health systems.