Monitoring Stroke Progression: In Vivo Imaging of Cortical Perfusion, Blood—Brain Barrier Permeability and Cellular Damage in the Rat Photothrombosis Model | Zendy

Karl Schoknecht | Zendy; Ofer Prager | Zendy; Udi Vazana | Zendy; Lyna Kamintsky | Zendy; Denise Harhausen | Zendy; Marietta Zille | Zendy; Lena Figge | Zendy; Yoash Chassidim | Zendy; Eyk Schellenberger | Zendy; Richard J. Kovacs | Zendy; Uwe Heinemann | Zendy; Alon Friedman | Zendy

Open Access

Monitoring Stroke Progression: In Vivo Imaging of Cortical Perfusion, Blood—Brain Barrier Permeability and Cellular Damage in the Rat Photothrombosis Model

Author(s) -

Karl Schoknecht,

Ofer Prager,

Udi Vazana,

Lyna Kamintsky,

Denise Harhausen,

Marietta Zille,

Lena Figge,

Yoash Chassidim,

Eyk Schellenberger,

Richard J. Kovacs,

Uwe Heinemann,

Alon Friedman

Publication year - 2014

Publication title -

journal of cerebral blood flow and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.167

H-Index - 193

eISSN - 1559-7016

pISSN - 0271-678X

DOI - 10.1038/jcbfm.2014.147

Subject(s) - blood–brain barrier , ischemia , medicine , cerebral blood flow , brain ischemia , in vivo , brain damage , stroke (engine) , neuroscience , vascular permeability , pathology , anesthesia , central nervous system , biology , cardiology , mechanical engineering , microbiology and biotechnology , engineering

Focal cerebral ischemia is among the main causes of death and disability worldwide. The ischemic core often progresses, invading the peri-ischemic brain; however, assessing the propensity of the peri-ischemic brain to undergo secondary damage, understanding the underlying mechanisms, and adjusting treatment accordingly remain clinically unmet challenges. A significant hallmark of the peri-ischemic brain is dysfunction of the blood-brain barrier (BBB), yet the role of disturbed vascular permeability in stroke progression is unclear. Here we describe a longitudinal in vivo fluorescence imaging approach for the evaluation of cortical perfusion, BBB dysfunction, free radical formation and cellular injury using the photothrombosis vascular occlusion model in male Sprague Dawley rats. Blood-brain barrier dysfunction propagated within the peri-ischemic brain in the first hours after photothrombosis and was associated with free radical formation and cellular injury. Inhibiting free radical signaling significantly reduced progressive cellular damage after photothrombosis, with no significant effect on blood flow and BBB permeability. Our approach allows a dynamic follow-up of cellular events and their response to therapeutics in the acutely injured cerebral cortex.

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