Differential Changes of Glutathione Levels in Astrocytes and Neurons in Ischemic Brains by Two-Photon Imaging | Zendy

Denis E. Bragin | Zendy; Bo Zhou | Zendy; Prabhu Ramamoorthy | Zendy; Wolfgang Müller | Zendy; John A. Connor | Zendy; Honglian Shi | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Differential Changes of Glutathione Levels in Astrocytes and Neurons in Ischemic Brains by Two-Photon Imaging

Author(s) -

Denis E. Bragin,

Bo Zhou,

Prabhu Ramamoorthy,

Wolfgang Müller,

John A. Connor,

Honglian Shi

Publication year - 2010

Publication title -

journal of cerebral blood flow and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.167

H-Index - 193

eISSN - 1559-7016

pISSN - 0271-678X

DOI - 10.1038/jcbfm.2010.9

Subject(s) - penumbra , glutathione , astrocyte , neuroscience , chemistry , two photon excitation microscopy , brain ischemia , ischemia , biophysics , medicine , biology , central nervous system , biochemistry , physics , fluorescence , optics , enzyme

Using two-photon imaging techniques with monochlorobimane as a glutathione (GSH) probe, we investigated GSH levels in both core and penumbra regions of an ischemic brain after middle cerebral artery occlusion. We found that the GSH level significantly decreased in the ischemic core, but increased significantly in the penumbra. Furthermore, we observed a differential change of the GSH levels in neurons and astrocytes in the penumbra. The GSH level in neurons increased significantly whereas it decreased slightly in astrocytes in the penumbra. These findings reveal critical region and cell type-dependent changes of the GSH level in an ischemic brain.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research