Glycogen Metabolism as a Marker of Astrocyte Differentiation | Zendy

JF Brunet | Zendy; Igor Allaman | Zendy; Pierre J. Magistretti | Zendy; Luc Pellerin | Zendy

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Glycogen Metabolism as a Marker of Astrocyte Differentiation

Author(s) -

JF Brunet,

Igor Allaman,

Pierre J. Magistretti,

Luc Pellerin

Publication year - 2009

Publication title -

journal of cerebral blood flow and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.167

H-Index - 193

eISSN - 1559-7016

pISSN - 0271-678X

DOI - 10.1038/jcbfm.2009.207

Subject(s) - ciliary neurotrophic factor , leukemia inhibitory factor , astrocyte , glycogen , glial fibrillary acidic protein , biology , cellular differentiation , fetal bovine serum , chemistry , neurotrophic factors , microbiology and biotechnology , endocrinology , biochemistry , interleukin 6 , cell , immunology , immunohistochemistry , central nervous system , cytokine , receptor , gene

Glycogen is a hallmark of mature astrocytes, but its emergence during astrocytic differentiation is unclear. Differentiation of E14 mouse neurospheres into astrocytes was induced with fetal bovine serum (FBS), Leukemia Inhibitory Factor (LIF), or Ciliary Neurotrophic Factor (CNTF). Cytochemical and enzymatic analyses showed that glycogen is present in FBS- or LIF- but not in CNTF-differentiated astrocytes. Glycogenolysis was induced in FBS- and LIF-differentiated astrocytes but glycogen resynthesis was observed only with FBS. Protein targeting to glycogen mRNA expression appeared with glial fibrillary acidic protein and S100β in FBS and LIF conditions but not with CNTF. These results show that glycogen metabolism constitutes a useful marker of astrocyte differentiation.

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