Open AccessIncreased F1/GAP-43 mRNA Accumulation in Gerbil Hippocampus after Brain Ischemia
Author(s) -
Masayoshi Tagaya,
Tomohiro Matsuyama,
Hitoshi Nakamura,
Ryuji Hata,
Souichiro Shimizu,
Hiroshi Kiyama,
Masayasu Matsumoto,
Minoru Sugita
Publication year - 1995
Publication title -
journal of cerebral blood flow and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.167
H-Index - 193
eISSN - 1559-7016
pISSN - 0271-678X
DOI - 10.1038/jcbfm.1995.140
Subject(s) - gerbil , ischemia , hippocampal formation , in situ hybridization , neuroscience , biology , granule (geology) , hippocampus , dentate gyrus , messenger rna , pathology , medicine , gene , biochemistry , paleontology
To assess whether ischemia could induce GAP-43 mRNA expression, we performed in situ hybridization in gerbil brains that had been subjected to 5 min of global ischemia. In control dentate granule cells, little hybridization was detected in contrast to the intense signal generated by pyramidal neurons of the adult hippocampal formation. After ischemia, we detected a robust GAP-43 signal over hippocampal granule cells at 3 h of reperfusion, persisting through 7 days, and disappearing by 14 days. This demonstrated GAP-43 gene induction after ischemia, and suggests that GAP-43 may be involved in reactive events, including fiber sprouting and synaptic reorganization, that follow ischemia.