In vitro Model of Hypoxia: Basic Fibroblast Growth Factor Can Rescue Cultured CNS Neurons from Oxygen-Deprived Cell Death | Zendy

Yukio Akaneya | Zendy; Yasushi Enokido | Zendy; Mitsuo Takahashi | Zendy; Hiroshi Hatanaka | Zendy

Open Access

In vitro Model of Hypoxia: Basic Fibroblast Growth Factor Can Rescue Cultured CNS Neurons from Oxygen-Deprived Cell Death

Author(s) -

Yukio Akaneya,

Yasushi Enokido,

Mitsuo Takahashi,

Hiroshi Hatanaka

Publication year - 1993

Publication title -

journal of cerebral blood flow and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.167

H-Index - 193

eISSN - 1559-7016

pISSN - 0271-678X

DOI - 10.1038/jcbfm.1993.130

Subject(s) - hypoxia (environmental) , hippocampal formation , basic fibroblast growth factor , programmed cell death , in vitro , biology , nerve growth factor , endocrinology , microbiology and biotechnology , medicine , cell culture , cell , neuroscience , growth factor , oxygen , chemistry , apoptosis , biochemistry , receptor , organic chemistry , genetics

We established an in vitro hypoxia model and investigated the protective effect of basic fibroblast growth factor (bFGF) against neuronal cell death caused by hypoxia. Hippocampal neurons obtained from rats on embryonic day (E) 17 and 20 and on postnatal day (P) 4 were cultured for 6-24 h in an oxygen-deprived state. This in vitro hypoxia study showed that the cultured neurons were sensitive to the oxygen deprivation. The cultured P4 rat hippocampal neurons seemed to be weaker in the hypoxia condition than those of E17 and E20 rats, suggesting that the cultured postnatal cells might be sensitive to hypoxia. bFGF, but not nerve growth factor, prevented the neuronal cell death caused by hypoxia in a dose-dependent manner.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research