Regional Imaging of Brain Tissue Calcium Ions Using Aequorin | Zendy

Kenji Hashimoto | Zendy; Haruhiko Kikuchi | Zendy; Masatsune Ishikawa | Zendy; Shuichi Kobayashi | Zendy

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Regional Imaging of Brain Tissue Calcium Ions Using Aequorin

Author(s) -

Kenji Hashimoto,

Haruhiko Kikuchi,

Masatsune Ishikawa,

Shuichi Kobayashi

Publication year - 1992

Publication title -

journal of cerebral blood flow and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.167

H-Index - 193

eISSN - 1559-7016

pISSN - 0271-678X

DOI - 10.1038/jcbfm.1992.42

Subject(s) - aequorin , photoprotein , calcium , bioluminescence , reagent , chemistry , ion , brain tissue , biophysics , biochemistry , anatomy , biology , organic chemistry

To investigate regional changes in calcium ion concentrations, we developed a new histochemical method using aequorin, a calcium ion-sensitive photoprotein. In this method, reagent film containing aequorin was made and an unfixed slice of frozen brain 16 μm thick was placed on it. Tissue calcium ions permeated the reagent layer and the bioluminescence of aequorin-calcium ions was recorded photographically with high spatial resolution. There was a close linear relationship ( r = 0.903) between the optical density of the bioluminescent images and the logarithmic values of the tissue calcium ion concentration. Using this method, we could visualize the regional tissue calcium ion distribution in pathological states in rat brains.

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