Open AccessMeasurement of Cerebral Monoamine Oxidase B Activity Using L-[11C]Deprenyl and Dynamic Positron Emission Tomography
Author(s) -
Adriaan A. Lammertsma,
C. J. Bench,
Gary Price,
J. E. Cremer,
Sajinder K. Luthra,
David R. Turton,
Nolan Wood,
R. S. J. Frackowiak
Publication year - 1991
Publication title -
journal of cerebral blood flow and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.167
H-Index - 193
eISSN - 1559-7016
pISSN - 0271-678X
DOI - 10.1038/jcbfm.1991.103
Subject(s) - positron emission tomography , monoamine oxidase b , monoamine oxidase , selegiline , monoamine oxidase a , nuclear magnetic resonance , nuclear medicine , physics , chemistry , medicine , enzyme , disease , parkinson's disease
A tracer kinetic procedure was developed for the measurement of monoamine oxidase type B (MAO-B) activity using L-[ 11 C]deprenyl and positron emission tomography (PET). The kinetic model consisted of two tissue compartments with irreversible binding to the second compartment (three rate constants). In addition, a blood volume component was included. Special attention was given to the accurate measurement of the plasma and whole blood input functions. The method was applied to the measurement of the dose-response curve of a reversible MAO-B inhibitor (Ro 19–6327). From the results, it followed that the rate constant for irreversible binding ( k 3 ) appeared to be a better index of MAO-B activity than the net influx constant K i Furthermore, regional analysis demonstrated that K i but not k 3 , was flow dependent. This implies that full kinetic analysis is required for an accurate assessment of MAO-B activity.
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